Induction of interferon synthesis by synthetic double-stranded polynucleotides

Induction of interferon synthesis by synthetic double-stranded polynucleotides.

It has been shown recently that double-stranded ribonucleic acid (RNA) from different sources stimulates the release of interferon into the circulatory system when injected intravenously into rabbits. The same RNA preparations protected mice from viral infections and prevented the viral cytopathic effect in cultures of rabbit kidney cells (A. K. Field et al., Proc. Natl. Acad. Sci. U.S. 58:2102...

Double-Stranded Polynucleotides as Interferon Inducers

A discussion of factors considered influential in making a polynucleotide an efficient inducer of interferon was presented. These factors were double-strandedness of the polynucleotides, the sugar moiety of the polynucleotides, thermal stability, resistance to enzymatic degradation, and molecular size of the polynucloetides. Recent developments concerning interferon induction during virus infec...

Interferon production by double-stranded RNA: a comparison of induction by reovirus to that by a synthetic double-stranded polynucleotide.

Specific Murine B-cell Activation by Synthetic Single- and Double-stranded Polynucleotides

The synthetic single- and double-stranded polynucleotides, poly I, poly C, and poly I.C, were shown to induce thymidine incorporation in six inbred strains of murine spleen cells. This stimulation was shown to be secondary to B-cell activation and not due to contamination of the polynucleotides with bacterial lipopolysaccharide (LPS). The ability of poly I.C to act as a B-cell mitogen, in addit...

Mouse hepatitis virus does not induce Beta interferon synthesis and does not inhibit its induction by double-stranded RNA.

Mouse hepatitis virus (MHV) does not induce interferon (IFN) production in fibroblasts or bone marrow-derived dendritic cells. In this report, we show that the essential IFN-beta transcription factors NF-kappaB and IFN regulatory factor 3 are not activated for nuclear translocation and gene induction during infection. However, MHV was unable to inhibit the activation of these factors and subseq...